Both strands utilized the same set of interior primers like in respective PCR assays
2.cuatro.step 3. Sequencing out of DNA
Getting DNA sequencing, the latest amplification circumstances obtained from new PCR reactions was indeed filtered that have the fresh Split II PCR and Gel Package (Bioline, London, UK) and you can sequenced from inside the a keen Avant 3100 sequencer (Applied Biosystem, Waltham, MA, USA). DNA sequencing was performed by an effective sequencing business during the School away from Veterinarian Medication, Kosice, Slovakia. This new gotten DNA sequences in the partial bg and tpi family genes was basically compared to the resource sequences of Grams. (suite…)
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Both strands utilized the same set of interior primers like in respective PCR assays